Kjeldahl method (nitrogen estimation) (%N = (1.4 × Normality of acid × Volume of acid neutralized by NH₃) / mass of organic)

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%N = (1.4 × Normality of acid × Volume of acid neutralized by NH₃) / mass of organic compound

What each symbol means

SymbolWhat it stands for
WMass of organic compound taken (g)
1.4Derived from 14 (atomic mass of N) × 100 / 1000 — converts milliequivalents to percentage
N_acidNormality of the standard acid (H₂SO₄)
V_acidVolume of acid (mL) consumed by evolved NH₃

When to use this

Does NOT work for compounds where N is in a ring (pyridine, quinoline), nitro groups (–NO₂), azo groups (–N=N–), hydrazine, or N bonded to N/O. These groups don't fully convert to (NH₄)₂SO₄ during digestion.